Vivaspin 500, MWCO 3 - 100 kDa

Pack size 25

Vivaspin 500 contains 25 disposable Vivaspin™ 500 ultrafiltration devices with either 3,000, 5,000, 10,000, 30,000, 50,000 or 100,000 molecular weight cut off (MWCO) polyethersulphone (PES). 

Vivaspin 500 is suitable for sample volumes of 100-500 μl. Vivaspin 500 can be used in a benchtop fixed angle rotor, accepting 2.2 ml centrifuge tubes.

Vivaspin spin columns are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.

• High recovery rates – Up to 30-fold concentration of the sample can be achieved, with recovery of the target molecule typically exceeding 95%.
• Sample protection – the patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
• Ease-of-use – one-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
• pH compatibility – for a broad range of pH 1 to 9.
• High flow rates – and minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
• Minimal protein adsorption – thanks to the Vivaspin polycarbonate tube design.

Spin Column Protein Concentration – How Does it Work?

Vivaspin sample concentrators cater to scientists working with sample volumes ranging from 100 μl to 20 ml, with a range of MWCO values from Mr 3 000 to 100 000.

An upper compartment contains sample and the lower compartment separated by a semipermeable membrane. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.

Tip: To maximize recovery of your sample, select a MWCO at least 50% smaller than the molecular size of the target molecule.